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KMID : 0379119860140030201
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Korean Journal of Mycology
1986 Volume.14 No. 3 p.201 ~ p.207
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Induction of Ribosomal Ribonuclease during Catabolic Repression in Saccharomyces uvarum
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Abstract
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In order to study subcellular locality and characteristics of ribonuclease in Saccharomyces uvarum, subcelllar fractions 45,000 x g pellet fraction, post ribosomal fraction and ribosome fraction were extracted during late log, stationary phase and sugar starvation conditions. Ribonuclease activity was significantly increased in ribosomal fraction under stationary and sugar starvation conditions. Ribosomal ribonuclease was extracted by EDTA plus streptomycin sulfate sad ammonium sulfate precipitation. The amount of ribosome in stationary and sugar starvation condition was decreased three to six fold as compared to that in the early log phase. The end products of ribosomal ribonuclease were detected by thin layer chromatography. It is postulated that the increase of ribosomal ribonuclease activity under sugar starvation results from 5´-rRNase, while the increase of rRNase activity under stationary phase results from 3´-rRNase.
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